Journal: Cell reports

Article Title: HIV-1 Vpr drives a tissue residency-like phenotype during selective infection of resting memory T cells.

doi: 10.1016/j.celrep.2022.110650

Figure Lengend Snippet: Figure 4. Incoming Vpr is sufficient to drive TRM induction in resting memory CD4+ T cells (A) CD69 (left) and CD69/CXCR6 (right) co-expression in response to IL-7 in the presence of integrase inhibitor raltegravir (n = 6). (B) Quantification of integrated provirus and 2LTR circles in FACS-sorted target CD4+ memory T cells after 72 h of cell-to-cell spread in the presence or absence of raltegravir. (C) Schematic depicting the viruses and VLPs used in (D–H). (D) Western blot showing Vpr packaging into virions of HIV-1 WTPM, VprPM, WT, DVpr, and DVpr complemented with FLAG-tagged Vpr in trans (DVpr+Vprtrans). (E) CD69 (left) and CD69/CXCR6 (right) upregulation in response to IL-7 on Gag+ resting memory CD4+ T cells at 72 h infected with the indicated HIV-1 viruses (n = 8). (F) Expression of CD69 (left) and CD69/CXCR6 (right) in response to IL-7 on Gag+ resting memory CD4+ T cells at 72 h post spinoculation of HIV-1 WT, Vpr, and DVpr+Vprtrans (n = 10). (G) Western blot showing packaging of FLAG-tagged Vpr into Env-VLPs or full-length HIV-1 WT or DVpr. (H) Expression of CD69 (left) and CD69/CXCR6 (right) in response to IL-7 on Gag+ resting memory CD4+ T cells at 72 h post spinoculation of Env-VLPs with or without Vpr (n = 5). All measurements were made after 72 h or at the indicated time post co-culture or spinoculation. Data are the mean ± SEM. Paired two-tailed t test or one-way ANOVA with Bonferroni or Dunnett’s post test was used. *p < 0.05; **p < 0.01; ***p < 0.001; n.s., not significant. EV, empty vector.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HIV-1 pNL4.3 Vpr S79A A. Reuschl This study HIV-1 pNL4.3 Vpr R80A A. Reuschl This study Biological samples PBMCs isolated from buffy coats from healthy donors UK NHS Blood and Transplant Service N/A Tonsillar tissue from elective tonsillectomy Imperial College Infectious Diseases Biobank N/A Lymph nodes obtained from field surgery of participants undergoing surgery for diagnostic purposes and/or complications of inflammatory lung disease University of KwaZulu-Natal N/A Human Serum from humanmale AB plasma Sigma-Aldrich Cat# H4522-20ML Chemicals, peptides, and recombinant proteins Phytohemagglutinin-L (PHA-L) Sigma Cat# 11249738001 Interleukin-2 (Human, rDNA derived) CFAR Cat# 86/500 Fugene 6 Transfection Reagent Promega Cat# E2691 DNase I Sigma Cat# DN25-100MG CellTraceTM Far Red Cell Proliferation Kit ThermoFisher Cat# C34564 Fixable Viability Dye eFluorTM 450 ThermoFisher Cat# 65-0863-14 Recombinant human IL-7 Miltenyi Biotec Cat# 130-095-362 Recombinant Human IL-15 Peptrotech Cat# 200-15 Recombinant Human IL-12 p70 Peprotech Cat# 200-12 Recombinant Human TGF-b1 Peprotech Cat# 100-21C T20 CFAR Cat# 0984 Efavirenz CFAR Cat# 0977 Raltegravir CFAR Cat# 0980 Ruxolitinib Selleckchem Cat# S1378 Zombie NIRTM Fixable Viability Kit Biolegend Cat# 423106 Zombie UVTM Fixable Viability Kit Biolegend Cat# 423108 Zombie AquaTM Fixable Viability Kit Biolegend Cat# 423102 Super Bright Staining Buffer ThermoFisher Cat# SB-4400 Brefeldin A Solution Biolegend Cat# 420601 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# P1585-1MG Ionomycin Sigma-Aldrich Cat# I9657-1MG Intracellular Staining Permeabilization Wash Buffer Biolegend Cat# 421002 FOXP3 Fix/Perm Buffer Set Biolegend Cat# 421403 True-PhosTM Perm Buffer Biolegend Cat# 425401 RLT Buffer (RNeasy Lysis Buffer) Qiagen Cat# 79216 b-mercaptoethanol (Sigma-Aldrich) Sigma-Aldrich Cat# M3148 Phusion Hot Start Flex DNA Polymerase NewEngland Biolabs Cat# M0535L TaqManTM Master-Mix ThermoFisher Cat# 4369016 SuperScriptTM IV Reverse Transcriptase ThermoFisher Cat# 18090050 Fast SYBRTM Green Master Mix Applied Biosystems Cat# 4385612 Hoechst33342 ThermoFisher Cat# H3570 Critical commercial assays MojoSortTM Human CD4 T Cell Isolation Kit Biolegend Cat# 480010 CD45RA MicroBeads, human Miltenyi Biotec Cat# 130-045-901 (Continued on next page) Cell Reports 39, 110650, April 12, 2022 e3

Techniques: Expressing, Western Blot, Infection, Co-Culture Assay, Two Tailed Test, Plasmid Preparation

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet:

Article Snippet: Anti-Human S100A8 (REA917) PE , Miltenyi Biotec , 130-115-253; RRID: AB_2726963.

Techniques: Purification, Recombinant, Staining, cDNA Synthesis, Gene Expression, Software, Microscopy

Journal: Experimental neurology

Article Title: Recent Advances in the Development of Immunotherapies for Tauopathies

doi: 10.1016/j.expneurol.2010.10.007

Figure Lengend Snippet: Studies Investigating Tau Immunization

Article Snippet: In the next section we discuss the findings of this important report by Boimel et al and how it compares with other reports. table ft1 table-wrap mode="anchored" t5 caption a7 ANIMAL MODEL ANTIGEN/ IMMUNOGEN ADJUVANT IMMUNIZATION OUTCOME REFERENCE C57Bl/6 50μg recombinant human tau protein (i.p) CFA and PT ACTIVE Anti-tau antibodies detected in serum however immunized animals displayed severe inflammation, axonal injury and loss. ( Rosenmann et al., 2006 ) P301L Tau Tau peptide 379–408, phosphorylated at Ser396/404 (s.c) Alum ACTIVE Anti-tau antibodies detected in CNS bound to pathological forms of tau.

Techniques: Adjuvant, Recombinant, Mutagenesis

Journal: Investigative ophthalmology & visual science

Article Title: Mefunidone Inhibits Inflammation, Oxidative Stress, and Epithelial-Mesenchymal Transition in Lens Epithelial Cells.

doi: 10.1167/iovs.65.14.17

Figure Lengend Snippet: FIGURE 5. Expression changes of CXCL1, S100A9, COX-2, and G-CSF in capsular bags 24 hours after extracapsular lens extraction combined with anterior chamber injection of MFD. At 24 hours after ECLE, the levels of CXCL1, S100A9, COX-2, and G-CSF were significantly increased, which were effectively mitigated by MFD (scale bar: 20 μm). e, remnant lens epithelial cells/lens cells; lc, lens capsule.

Article Snippet: Mefunidone is provided by the Xiangya School of Pharmaceutical Sciences, Central South University; Dulbecco’s Modified Eagle Medium (DMEM) with high glucose (cat. C11995500; Gibco, California, USA); fetal bovine serum (FBS) (cat. FSP500; ExCell, Suzhou, China); penicillin– streptomycin (cat. C0222; Beyotime, Shanghai, China); recombinant human TGF-β2 protein (cat. HZ-1092; Proteintech, Chicago, USA); Trizol reagent (cat. 15596018; Thermo Scientific, Massachusetts, USA); cDNA Synthesis SuperMix for qPCR (cat. 11141; Yeasen, Shanghai, China); SYBR Green Master Mix (cat. 11202; Yeasen); Cell Counting Kit-8 (CCK8) (cat. EK-5103; ECOTOP SCIENTIFIC, Guangzhou, China); Lipid Peroxidation MDA Assay Kit (cat. S0131S; Beyotime); Reactive Oxygen Species Assay Kit (cat. S0033S; Beyotime); reduced glutathione (GSH) and oxidized glutathione disulfide (GSSG) Assay Kit (cat. S0053; Beyotime); GSH Kit (cat. BC1175; Solarbio, Beijing, China); 24-well transwell chambers (cat. 3422; Corning, Corning, NY, USA); NcmBlot Rapid Transfer Buffer (cat. WB4600; NCM Biotech, Suzhou, China); SDS-PAGE Loading Buffer (cat. WB2001; NCM Biotech); E-cadherin antibody (cat. 3195; Cell Signaling Technology, Boston, USA); SMAD 2/3 antibody (cat. 8685; Cell Signaling Technology); anti–fibronectin antibody (cat. ab2413; abcam, Massachusetts, USA); Collagen I antibody (cat. A1352; ABclonal, Wuhan, China); S100A9 antibody (cat. 9842; ABclonal); anti–G-CSF antibody (cat. ab181053; abcam); anti-CXCL1 antibody (cat. 12335-1-AP; Proteintech); anti–COX-2 antibody (cat. ab179800; abcam); anti-SMAD3 (phospho S423+S425) antibody (cat. ab52903; abcam); phospho-SMAD2 antibody (cat. 18338; Cell Signaling Technology); anti–α-SMA antibody (cat. ab124964; abcam); p44/42 MAPK (Erk1/2) antibody (cat. 4695; Cell Signaling Technology); phospho-p44/42 MAPK (Erk1/2) antibody (cat. 9101; Cell Signaling Technology); vimentin antibody (cat. A19608; ABclonal); anti-rabbit IgG Alexa Fluor 488 (cat. 4412; Cell Signaling Technology); GAPDH (cat. 60004-1-Ig; Proteintech); HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) (cat. SA00001-1; Proteintech); HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) (cat. SA00001-2; Proteintech); and TUNEL Assay Apoptosis Detection Kit (cat. T6013; UElandy, Suzhou, China).

Techniques: Expressing, Extraction, Injection

Journal: Investigative ophthalmology & visual science

Article Title: Mefunidone Inhibits Inflammation, Oxidative Stress, and Epithelial-Mesenchymal Transition in Lens Epithelial Cells.

doi: 10.1167/iovs.65.14.17

Figure Lengend Snippet: FIGURE 6. Mefunidone alleviated inflammation and oxidative stress in vivo. (A, B) Western blot and quantitative data of G-CSF and S100A9 in capsular bags after ECLE with or without anterior chamber injection of MFD. GAPDH was used as a loading control. (C) The MDA level in capsular bags of different times with or without anterior chamber injection of MFD. (D) The GSH level in capsular bags at 24 hours after ECLE with or without anterior chamber injection of MFD. (E) qRT-PCR analysis of G-CSF, S100A9, CXCL1, and COX-2 in capsular bags after ECLE with or without anterior chamber injection of MFD. Values are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: Mefunidone is provided by the Xiangya School of Pharmaceutical Sciences, Central South University; Dulbecco’s Modified Eagle Medium (DMEM) with high glucose (cat. C11995500; Gibco, California, USA); fetal bovine serum (FBS) (cat. FSP500; ExCell, Suzhou, China); penicillin– streptomycin (cat. C0222; Beyotime, Shanghai, China); recombinant human TGF-β2 protein (cat. HZ-1092; Proteintech, Chicago, USA); Trizol reagent (cat. 15596018; Thermo Scientific, Massachusetts, USA); cDNA Synthesis SuperMix for qPCR (cat. 11141; Yeasen, Shanghai, China); SYBR Green Master Mix (cat. 11202; Yeasen); Cell Counting Kit-8 (CCK8) (cat. EK-5103; ECOTOP SCIENTIFIC, Guangzhou, China); Lipid Peroxidation MDA Assay Kit (cat. S0131S; Beyotime); Reactive Oxygen Species Assay Kit (cat. S0033S; Beyotime); reduced glutathione (GSH) and oxidized glutathione disulfide (GSSG) Assay Kit (cat. S0053; Beyotime); GSH Kit (cat. BC1175; Solarbio, Beijing, China); 24-well transwell chambers (cat. 3422; Corning, Corning, NY, USA); NcmBlot Rapid Transfer Buffer (cat. WB4600; NCM Biotech, Suzhou, China); SDS-PAGE Loading Buffer (cat. WB2001; NCM Biotech); E-cadherin antibody (cat. 3195; Cell Signaling Technology, Boston, USA); SMAD 2/3 antibody (cat. 8685; Cell Signaling Technology); anti–fibronectin antibody (cat. ab2413; abcam, Massachusetts, USA); Collagen I antibody (cat. A1352; ABclonal, Wuhan, China); S100A9 antibody (cat. 9842; ABclonal); anti–G-CSF antibody (cat. ab181053; abcam); anti-CXCL1 antibody (cat. 12335-1-AP; Proteintech); anti–COX-2 antibody (cat. ab179800; abcam); anti-SMAD3 (phospho S423+S425) antibody (cat. ab52903; abcam); phospho-SMAD2 antibody (cat. 18338; Cell Signaling Technology); anti–α-SMA antibody (cat. ab124964; abcam); p44/42 MAPK (Erk1/2) antibody (cat. 4695; Cell Signaling Technology); phospho-p44/42 MAPK (Erk1/2) antibody (cat. 9101; Cell Signaling Technology); vimentin antibody (cat. A19608; ABclonal); anti-rabbit IgG Alexa Fluor 488 (cat. 4412; Cell Signaling Technology); GAPDH (cat. 60004-1-Ig; Proteintech); HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) (cat. SA00001-1; Proteintech); HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) (cat. SA00001-2; Proteintech); and TUNEL Assay Apoptosis Detection Kit (cat. T6013; UElandy, Suzhou, China).

Techniques: In Vivo, Western Blot, Injection, Control, Quantitative RT-PCR

Journal: Investigative ophthalmology & visual science

Article Title: Mefunidone Inhibits Inflammation, Oxidative Stress, and Epithelial-Mesenchymal Transition in Lens Epithelial Cells.

doi: 10.1167/iovs.65.14.17

Figure Lengend Snippet: FIGURE 9. Mefunidone attenuated inflammation and oxidative stress induced by H2O2 in LECs. (A) CCK-8 assay of LECs treated with 0 to 600 μmol/L H2O2 for 6 hours. CCK-8 results showed that H2O2 inhibited cell viability in a concentration-dependent manner, and the inhibition efficiency reached 50% when the concentration was 400 μmol/L. (B) The MDA level in LECs of different groups. MDA detection results demonstrated an increase in MDA content in the H2O2-induced model group, which was inhibited by MFD. (C) The GSH level in LECs of different groups. An obvious decrease in GSH was detected in the H2O2-induced group, and MFD restored its expression. (D) DCFH-DA probe was used to detect the intracellular ROS levels in LECs. The green fluorescence intensity indicated the level of intracellular ROS (scale bar: 100 μm). (E) qRT-PCR analysis of CXCL1, S100A9, COX-2, and G-CSF in LECs pretreated with or without 100 μmol/L MFD overnight with or without 400 μmol/L H2O2 for another 6 hours.

Article Snippet: Mefunidone is provided by the Xiangya School of Pharmaceutical Sciences, Central South University; Dulbecco’s Modified Eagle Medium (DMEM) with high glucose (cat. C11995500; Gibco, California, USA); fetal bovine serum (FBS) (cat. FSP500; ExCell, Suzhou, China); penicillin– streptomycin (cat. C0222; Beyotime, Shanghai, China); recombinant human TGF-β2 protein (cat. HZ-1092; Proteintech, Chicago, USA); Trizol reagent (cat. 15596018; Thermo Scientific, Massachusetts, USA); cDNA Synthesis SuperMix for qPCR (cat. 11141; Yeasen, Shanghai, China); SYBR Green Master Mix (cat. 11202; Yeasen); Cell Counting Kit-8 (CCK8) (cat. EK-5103; ECOTOP SCIENTIFIC, Guangzhou, China); Lipid Peroxidation MDA Assay Kit (cat. S0131S; Beyotime); Reactive Oxygen Species Assay Kit (cat. S0033S; Beyotime); reduced glutathione (GSH) and oxidized glutathione disulfide (GSSG) Assay Kit (cat. S0053; Beyotime); GSH Kit (cat. BC1175; Solarbio, Beijing, China); 24-well transwell chambers (cat. 3422; Corning, Corning, NY, USA); NcmBlot Rapid Transfer Buffer (cat. WB4600; NCM Biotech, Suzhou, China); SDS-PAGE Loading Buffer (cat. WB2001; NCM Biotech); E-cadherin antibody (cat. 3195; Cell Signaling Technology, Boston, USA); SMAD 2/3 antibody (cat. 8685; Cell Signaling Technology); anti–fibronectin antibody (cat. ab2413; abcam, Massachusetts, USA); Collagen I antibody (cat. A1352; ABclonal, Wuhan, China); S100A9 antibody (cat. 9842; ABclonal); anti–G-CSF antibody (cat. ab181053; abcam); anti-CXCL1 antibody (cat. 12335-1-AP; Proteintech); anti–COX-2 antibody (cat. ab179800; abcam); anti-SMAD3 (phospho S423+S425) antibody (cat. ab52903; abcam); phospho-SMAD2 antibody (cat. 18338; Cell Signaling Technology); anti–α-SMA antibody (cat. ab124964; abcam); p44/42 MAPK (Erk1/2) antibody (cat. 4695; Cell Signaling Technology); phospho-p44/42 MAPK (Erk1/2) antibody (cat. 9101; Cell Signaling Technology); vimentin antibody (cat. A19608; ABclonal); anti-rabbit IgG Alexa Fluor 488 (cat. 4412; Cell Signaling Technology); GAPDH (cat. 60004-1-Ig; Proteintech); HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) (cat. SA00001-1; Proteintech); HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) (cat. SA00001-2; Proteintech); and TUNEL Assay Apoptosis Detection Kit (cat. T6013; UElandy, Suzhou, China).

Techniques: CCK-8 Assay, Concentration Assay, Inhibition, Expressing, Fluorescence, Quantitative RT-PCR

Journal: Cell Reports Medicine

Article Title: Alarmin S100A8 imparts chemoresistance of esophageal cancer by reprogramming cancer-associated fibroblasts

doi: 10.1016/j.xcrm.2024.101576

Figure Lengend Snippet:

Article Snippet: Human S100A8/S100A9 ELISA Kit , Proteintech , Cat# KE00177.

Techniques: Virus, shRNA, Over Expression, Recombinant, Purification, Staining, Enzyme-linked Immunosorbent Assay, Sonication, Chromatin Immunoprecipitation, Software

Journal: Rheumatology and Immunology Research

Article Title: mRNA vaccine against fibroblast activation protein ameliorates murine models of inflammatory arthritis

doi: 10.2478/rir-2023-0013

Figure Lengend Snippet: Suppression of arthritis by cFAP protein vaccination. cFAP or OVA at 100 μg was mixed with alum adjuvant and injected subcutaneously into male DBA/1 mice. Three weeks later, Collagen-induced arthritis (CIA) was induced and development of arthritis was monitored clinically. Alum, aluminum containing adjuvant; cFAP, consensus fibroblast activation protein; ns, not significant; OVA, ovalbumin; *P < 0.05.

Article Snippet: Recombinant cFAP (custom produced by GenScript Biotech Corp. (Piscataway, NJ, USA) using the consensus FAP cDNA) was serial diluted at 1:2 in PBS containing 0.50% BSA and 0.05% Tween20 with top concentration of 2000 ng/mL to 2 ng/mL; and culture supernatant samples were incubated for 2 h at room temperature.

Techniques: Adjuvant, Injection, Activation Assay

Journal: Rheumatology and Immunology Research

Article Title: mRNA vaccine against fibroblast activation protein ameliorates murine models of inflammatory arthritis

doi: 10.2478/rir-2023-0013

Figure Lengend Snippet: Mouse cytomegalovirus (MCMV) vectored cFAP induced anti-FAP antibody response in vivo and amelioration of arthritis. Male DBA/1 mice were infected with MCMV vectored cFAP DNA (MCMV-cFAP) or wild-type MCMV (WT-MCMV). (A) Serum anti-cFAP and mouse FAP (mFAP) were detected by ELISA. Antibody titer was expressed as arbitrary unit by ratio of optical density of serum over that of standard monoclonal anti-FAP antibodies. (B) CIA was induced 3 weeks after MCMV infection and development of arthritis was monitored clinically. cFAP, consensus fibroblast activation protein; CIA, collagen-induced arthritis.

Article Snippet: Recombinant cFAP (custom produced by GenScript Biotech Corp. (Piscataway, NJ, USA) using the consensus FAP cDNA) was serial diluted at 1:2 in PBS containing 0.50% BSA and 0.05% Tween20 with top concentration of 2000 ng/mL to 2 ng/mL; and culture supernatant samples were incubated for 2 h at room temperature.

Techniques: In Vivo, Infection, Enzyme-linked Immunosorbent Assay, Activation Assay

Journal: Rheumatology and Immunology Research

Article Title: mRNA vaccine against fibroblast activation protein ameliorates murine models of inflammatory arthritis

doi: 10.2478/rir-2023-0013

Figure Lengend Snippet: Expression of cFAP using mRNA-LNP transfection in vitro. HEK239 cells were incubated with cFAP mRNA-LNP or Lipofectamine™ 3000 Transfection Reagent (L3K) at 2 μg per 5 x 10 5 cells. (A) Supernatants were harvested at 48 h post-transfection and cFAP were measured by a capture ELISA (showing 3 experiments and each condition was measured in triplicate). (B) In separate experiments, supernatants and cells were harvested at 24 and 48 h post-transfection for Western blotting. In some experiments, Golgi Plug was added for 5 h to block secretion of cFAP. mFAP, recombinant mouse FAP; cFAP, recombinant consensus FAP.

Article Snippet: Recombinant cFAP (custom produced by GenScript Biotech Corp. (Piscataway, NJ, USA) using the consensus FAP cDNA) was serial diluted at 1:2 in PBS containing 0.50% BSA and 0.05% Tween20 with top concentration of 2000 ng/mL to 2 ng/mL; and culture supernatant samples were incubated for 2 h at room temperature.

Techniques: Expressing, Transfection, In Vitro, Incubation, Enzyme-linked Immunosorbent Assay, Western Blot, Blocking Assay, Recombinant

Journal: Rheumatology and Immunology Research

Article Title: mRNA vaccine against fibroblast activation protein ameliorates murine models of inflammatory arthritis

doi: 10.2478/rir-2023-0013

Figure Lengend Snippet: Antibody production in mice immunized with cFAP mRNA-LNP. Male DBA/1 mice were injected intramuscularly with cFAP mRNA-LNP in 3 dose groups (1.5, 3 or 6 μg/mouse), or eGFP mRNA-LNP eGFP (1.5 μg/mouse) at week 0; at week 3, a booster cFAP mRNA-LNP (all at 1.5 μg/mouse) or eGFP mRNA-LNP (1.5 μg/mouse) was injected. Serum was collected weekly for measurement of antibodies to cFAP (A) and mFAP (B) as in (n = 5 in each group). cFAP, consensus fibroblast activation protein; EGFP, enhanced green fluorescent protein; LNP, lipid nanoparticle; PBS, phosphate buffered saline.

Article Snippet: Recombinant cFAP (custom produced by GenScript Biotech Corp. (Piscataway, NJ, USA) using the consensus FAP cDNA) was serial diluted at 1:2 in PBS containing 0.50% BSA and 0.05% Tween20 with top concentration of 2000 ng/mL to 2 ng/mL; and culture supernatant samples were incubated for 2 h at room temperature.

Techniques: Injection, Activation Assay, Saline

Journal: Rheumatology and Immunology Research

Article Title: mRNA vaccine against fibroblast activation protein ameliorates murine models of inflammatory arthritis

doi: 10.2478/rir-2023-0013

Figure Lengend Snippet: Suppression of arthritis by cFAP LNP-mRNA vaccine. Male DBA/1 mice were injected intramuscularly with cFAP mRNA-LNP (1.5 μg/mouse) or eGFP mRNA-LNP (1.5 μg/mouse) at week 0, then a booster injection was given in the same dose at week 3. Two weeks after the booster of cFAP LNP-mRNA, collagen-induced arthritis (CIA) or collagen antibody-induced arthritis (CAIA) was induced. Development of arthritis was assessed clinically (A-D) and by histology (E-G). CIA (A, B and E); CAIA (C, D, F). n = 6 in each group; *P < 0.01; **P < 0.05.

Article Snippet: Recombinant cFAP (custom produced by GenScript Biotech Corp. (Piscataway, NJ, USA) using the consensus FAP cDNA) was serial diluted at 1:2 in PBS containing 0.50% BSA and 0.05% Tween20 with top concentration of 2000 ng/mL to 2 ng/mL; and culture supernatant samples were incubated for 2 h at room temperature.

Techniques: Injection

Journal: Cell Reports Medicine

Article Title: Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors

doi: 10.1016/j.xcrm.2023.101374

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-S100A8 , ProteinTech , Cat#15792-1-AP; RRID: 10666315.

Techniques: Recombinant, Derivative Assay, Microarray, Enzyme-linked Immunosorbent Assay, Lysis, Blocking Assay, Staining, Transfection, Marker, Flow Cytometry, shRNA, Plasmid Preparation, Negative Control, Silver Staining, Bicinchoninic Acid Protein Assay, Selection, Extraction, Labeling, RNA Expression, Cell Culture, Software